The 4th Annual Course was held at the University of Rochester, the founding home of the
Annual Course in Cytometry. All participants attended every lab, with evening sessions
running until 11 pm.
Lectures
- What is flow cytometryKathy Muirhead
- Cytochemical probes for flow cytometryAlan Waggoner
- Anatomy of a flow cytometerPaul Horan
- Light scatter as a probe in cytometryJim Leary
- Single color immunofluorescence analysis (polyclonal and monoclonal antibodies)Noel Warner
- Two color immunofluorescence analysis (FITC & RITC)Mike Loken
- Analysis methods for immunofluorescence dataBruce Bagwell
- Rare event analysisJim Leary
- Combined DNA and immunofluorescence staining of mammalian cellsJohn Lehman
- Acridine orange methods for cell kinetic analysisZbigniew Darzynkiewicz
- Cell cycle analysisPhil Dean
- Chromosome analysis by flow cytometryScott Cram
- Special lecture — Application of flow cytometry to recombinant DNA methodologySam Latt
- Special lecture — Application of flow cytometry to the study of human lymphoid subsetsEllis Reinherz
Labs
All participants attended every lab. Evening sessions ran until 11 pm.
- Single color analysisNoel Warner
- Two color analysisMichael Loken
- Rare event analysisJim Leary
- Computer analysisBruce Bagwell
- Live-dead cell analysisEdith Lord
- Care & feeding of a laser; sorting (optional)Tom Schmitt & Paul Horan
